Performance should include
- explaining that PCR testing is a relatively quick procedure for analyzing DNA without the need for cloning and that it is commonly used for diagnosing genetic diseases
- following the steps for PCR testing
- reagent preparation
- equipment preparation
- proper loading of the gel
- proper use of the PCR thermocycler.
Process/Skill Questions:
- What is a nucleotide? What is the role of nucleotides in PCR testing?
- Why are amino acid sequences important in PCR?
- How can a DNA segment be replicated?
- What was the purpose of the Human Genome Project?
- At what temperature does denaturization occur?
- What buffer is used in PCR testing?
- What is the principle of a thermocycler?