Demonstration should include basic pipetting techniques, using autodelivery, volumetric, and transfer pipettes.
OSHA mandates no oral pipetting in any laboratory or clinical setting.
Process/Skill Questions:
- How is the principle of air displacement used in pipetting?
- How do air bubbles affect the accuracy of volume when pipetting?
- Why is selection of the appropriate pipette important in the delivery of fluid?
- What is the process of pipette calibration, and why is it important?