Demonstrate the procedure to run thin layer chromatography (TLC) tests.
DefinitionDemonstration should include
- using a TLC plate and other laboratory apparatus
- adding reagents to a series of known and/or questioned chemicals
- observing and describing the interaction
- documenting the results.
- What are the procedures for conducting TLC tests?
- What is the purpose of a TLC test?
- What types of materials can be tested using chromatography?
Related Standards of Learning
The student will demonstrate an understanding of scientific reasoning, logic, and the nature of science by planning and conducting investigations in which
- observations of living organisms are recorded in the lab and in the field;
- hypotheses are formulated based on direct observations and information from scientific literature;
- variables are defined and investigations are designed to test hypotheses;
- graphing and arithmetic calculations are used as tools in data analysis;
- conclusions are formed based on recorded quantitative and qualitative data;
- sources of error inherent in experimental design are identified and discussed;
- validity of data is determined;
- chemicals and equipment are used in a safe manner;
- appropriate technology including computers, graphing calculators, and probeware is used for gathering and analyzing data, communicating results, modeling concepts, and simulating experimental conditions;
- research utilizes scientific literature;
- differentiation is made among a scientific hypothesis, theory, and law;
- alternative scientific explanations and models are recognized and analyzed; and
- current applications of biological concepts are used.
The student will investigate and understand that experiments in which variables are measured, analyzed, and evaluated produce observations and verifiable data. Key concepts include
- designated laboratory techniques;
- safe use of chemicals and equipment;
- proper response to emergency situations;
- manipulation of multiple variables, using repeated trials;
- accurate recording, organization, and analysis of data through repeated trials;
- mathematical and procedural error analysis;
- mathematical manipulations including SI units, scientific notation, linear equations, graphing, ratio and proportion, significant digits, and dimensional analysis;
- use of appropriate technology including computers, graphing calculators, and probeware for gathering data, communicating results, and using simulations to model concepts;
- construction and defense of a scientific viewpoint; and
- the use of current applications to reinforce chemistry concepts.