# CTE Resource Center - Verso - Medical Laboratory Technology II Task 1509003495

CTE Resource Center - Verso

Virginia’s CTE Resource Center

Perform inoculation of patient specimen.

Definition

Performance should include plating on media that enables differentiation between gram negative and gram positive organisms. It should also include an explanation of media and their purposes:
  • CNA (colistin naladixic acid) agar isolates gram-positive bacteria.
  • EMB (eosin methylene blue) agar isolates gram-negative bacteria.
  • MacConkey agar isolates gram-negative bacteria.

Process/Skill Questions

  • How does CNA agar isolate gram-positive bacteria?
  • Which media would be useful in isolating E. coli? What components of the agar make this possible?

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