# CTE Resource Center - Verso - Medical Laboratory Technology II Task 1509003492

CTE Resource Center - Verso

Virginia’s CTE Resource Center

Perform microscopy and Gram stain preparation.


Performance should include
  • preparation of a Gram stain using crystal violet, Gram's iodine, decolorizer, and safranin
  • examination of a Gram-stained smear for gram-positive and gram-negative organisms.

Process/Skill Questions

  • Why is immersion oil used when examining a smear for bacteria?
  • What is the importance of using the correct order of reagents when performing a Gram stain?
  • How do gram-positive organisms appear on a Gram stain?
  • How do the gram-negative organisms appear on a Gram stain?

Related Standards of Learning



The student will demonstrate an understanding of scientific reasoning, logic, and the nature of science by planning and conducting investigations in which
  1. observations of living organisms are recorded in the lab and in the field;
  2. hypotheses are formulated based on direct observations and information from scientific literature;
  3. variables are defined and investigations are designed to test hypotheses;
  4. graphing and arithmetic calculations are used as tools in data analysis;
  5. conclusions are formed based on recorded quantitative and qualitative data;
  6. sources of error inherent in experimental design are identified and discussed;
  7. validity of data is determined;
  8. chemicals and equipment are used in a safe manner;
  9. appropriate technology including computers, graphing calculators, and probeware is used for gathering and analyzing data, communicating results, modeling concepts, and simulating experimental conditions;
  10. research utilizes scientific literature;
  11. differentiation is made among a scientific hypothesis, theory, and law;
  12. alternative scientific explanations and models are recognized and analyzed; and
  13. current applications of biological concepts are used.

Other Related Standards

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Medical Terminology


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Biomedical Laboratory Science